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Wailap Victor Ng

imageWailap Victor Ng, Ph.D.

Professor
Institute of Biotechnnology in Medicine
Institute of Bioinformatics
Department of Biotechnology and Lab Science in Medicine

Personal Web Site

Phone:  02-2826-7321 (office)

Fax:  02-2826-4092 (fax)

Email:  wvng@ym.edu.tw


Research Interests
Systems biology - Apply DNA microarray, mass spectrometry, 2-hybrid expression system, and bioinformatics technologies to (i) characterize the biomodules essential for cell survival upon environmental perturbations in Halobacterium sp. NRC-1 and Haloarcula marismortui and (ii) identify the new functions of more than 50% of all the predicted genes in these microorganisms.
Biomarker discovery - Identify cancer diagnostic markers and therapeutic targets using bioinformatics and mass spectrometry tools.
Technology development - EST database mining, mass spectrometry, and mass spectrometry data analysis system.

Publications
Genomics
1. Baliga, N.B., Bonneau, R., Facciotti, M.T., Pan, M., Glusman, G., Deutsch, E.W., Shannon, P., Chiu, Y., Weng, R.S., Gan, R.R., Hung, P., Date, S., Marcotte, E., Hood, L., Ng, W.V.Genome of Haloarcula marismortui – a halophilic archaeon from the Dead Sea. Genome Research 14:2221-34.
2. Goo, Y.A., Roach, J., Glusman, G., Baliga, N.S. , Deutsch, K., Pan, M., Kennedy, S.,DasSarma, S, Ng W.V., Hood L. 2004. Low-pass sequencing for microbial comparative genomics. BMC Genomics. 5:3-21.
3. Ng, W.V. et al. 2000. Genome sequence of Halobacterium sp. NRC-1. Proc Natl Acad Sci97:12176-81.
4. Ng, W.-L. et al. 1998. Snapshot of a large dynamic replicon in a halophilic archaeon:megaplasmid or minichromosome? Genome Res. 8:1131-41.

Proteomics
1. Goo, Y.A., Yi, E.C., Baliga, N.S. , Tao, W.A., Pan, M., Aebersold, R., Goodlett, D.R., Hood, L.,Ng, W.V. 2003. Proteomic Analysis of an Extreme Halophilic Archaeon, Halobacterium sp. NRC-1. Mol Cell Proteomics. 2:506-24.

Microbiology
1. Baliga, N.S. , Pan, M., Goo, Y.A., Yi, E.C., Goodlett, D.R., Dimitrov, K., Shannon , P.,Aebersold, R., Ng, W.V., Hood, L. 2002. Coordinate regulation of energy transduction modules in Halobacterium sp. analyzed by a global systems approach. Proc Natl Acad Sci U S A99:14913-8
2. Kennedy, S.P., Ng, W.V., Salzberg, SL., Hood, L., DasSarma, S. 2001. Understanding the adaptation of Halobacterium species NRC-1 to its extreme environment through computational analysis of its genome sequence. Genome Res. 11:1641-50.
3. Baliga, N.S. , Kennedy, S.P., Ng, W.V., Hood, L., DasSarma, S. 2001. Genomic and genetic dissection of an archaeal regulon. Proc Natl Acad Sci U S A 98:2521-5.
4. Peck, R.F., Echavarri-Erasun, C., Johnson, E.A., Ng, W.V., Kennedy, S.P., Hood, L.,DasSarma S., Krebs, M.P. 2001. brp and blh are required for synthesis of the retinal cofactor ofbacteriorhodopsin in Halobacterium salinarum. J. Biol. Chem 276:5739-44.
5. DasSarma, S., Kennedy, S.P., Berquist, B., Ng, W.V., Baliga, N.S., Spudich, J.L., Krebs, M.P., Eisen, J.A., Johnson, C.H., Hood, L. 2001. Genomic perspective on the photobiology ofHalobacterium species NRC-1, a phototrophic, phototactic, and UV-tolerant haloarchaeon.Photosyn. Res. 70:3-17.
6. Baliga, N.S. , Goo, Y.A., Ng, W.V., Hood, L., Daniels, C.J., and DasSarma, S. 2000. Is gene expression in Halobacterium NRC-1 regulated by multiple TBP and TFB transcription factors?Mol. Microbiol. 36:1184-5
7. Ng, W.-L., C.-F. Yang, J.T. Halladay, P. Arora, and S. DasSarma. 1995. Isolation of genomic and plasmid DNA from Halobacterium halobium. In S. DasSarma and E.M. Fleishmann (ed.), “Archaea: A laboratory manual - Halophiles”, Cold spring Harbor Laboratory press, p155-160.
8. Ng, W.-L., Arora, P., and DasSarma, S. 1994. Large deletion in class III gas vesicle-deficient mutants of Halobacterium halobium. System. Appl. Microbiol. 16: 560-568.
9. Bobovnikova, Y., Ng, W.-L., DasSarma, S., and Hackett, N.R. 1994. Restriction mapping the genome of Halobacterium halobium strain NRC-1. System. Appl. Microbiol. 16: 597-604.
10. Ng, W.-L. and DasSarma, S. 1993. Minimal replication origin of the 200 kilobase Halobacteriumplasmid pNRC100. J. Bacteriol. 175: 4584-4596.
11. Halladay, J.T, Ng, W.-L., and DasSarma, S. 1992. Genetic transformation of a halophilicarchaebacterium with a gas vesicle gene cluster restores its ability to float. Gene 119: 131-136.
12. Ng, W.-L., Kothakota, S., and DasSarma, S. 1991. Structure of the gas vesicle plasmid inHalobacterium halobium: inversion isomers, inverted repeats, and insertion sequences. J.Bacteriol. 173:1958 1964.
13. Ng, W.-L. and DasSarma ,S. 1991. Physical and genetic mapping of the unstable gas vesicle plasmid in Halobacterium halobium NRC-1. In F. Rodriguez-Valera (ed.), “General and applied aspects of halophilic microorganisms”, Plenum Press, New York , p.305-311.
14. Ng, W.-L., Halladay, J.T., and DasSarma, S. 1991. Isolation of large Halobacterium halobiumplasmids, p. 3.5.1-3.5.3. In E.M. Fleischmann, A.R. Place, F.T. Robb, and H.J. Schreier (ed.), Protocols for archaebacterial research. University of Maryland Baltimore.
15. J.G. Jones, Hackett, N.R.,. Halladay, J.T, Scothorn, D.J., Yang, C.-F., Ng, W.-L., andDasSarma, S. 1989. Analysis of insertion mutants reveals two new genes in the pNRC100 gas vesicle cluster of Halobacterium halobium. Nucleic Acids Res. 17:7785-7793.

Cancer
1. Schummer, M., Ng, W.V., Bumgarner, R.E., Nelson, P.S., Schummer, B., Bednarski, D.W.,Hassell, L., Baldwin , R.L., Karlan, B. Y., and Hood, L. 1999. Comparative hybridization of an array of 21,500 ovarian cDNAs for the discovery of genes overexpressed in ovarian carcinomas.Gene 238:375-85.
2. Huang, G.M., Ng, W.-L., Farkas, J., He, L., Liang, H.A., Gordon, D., Yu, J., and Hood, L. 1999. Prostate cancer expression profiling by cDNA sequencing analysis. Genomics 59:178-86.
3. Wang, K, Gan, L., Jeffery, E., Gayle, M., Gown, A.M., Skelly, M., Nelson, P.S., Ng, W.V.,Schummer, M., Hood, L., and Mulligan, J. 1999. Monitoring gene expression profile changes in ovarian carcinomas using cDNA microarray. Gene 229:101-8.
4. Nelson, P.S., Ng, W.-L., Schummer, M., True, L.D., Liu, A.L., Bumgarner, R., Ferguson , C.,Dimak, A., and Hood, L. An expressed-sequence-tag database of the human prostate: Sequence analysis of 1,168 cDNA clones. Genomics 47: 12-25, 1998.

Technologies
1. Nelson, P.S., Hawkins, V., Schummer, M., Bumgarner, R, Ng, W.-L., Ideker, T., Ferguson , C., and Hood, L. 1999 Negative selection: a method for obtaining low-abundance cDNAs using high-density cDNA clone arrays. Genet An al 15:209-15.
2. M. Schummer, Ng, W.-L., Nelson, P. S., Bumgarner, R.E, and Hood, L. An inexpensive and-held device for the construction of high density nucleic acid arrays. Biotechniques 23: 1087-1092, 1997.
3. Ng, W.-L., Schummer, M., Cirisano, F.D., Baldwin , R.E., Karlan, B.Y., and Hood, L. 1996. High-throughput plasmid mini preparation facilitated by micro-mixing. Nucleic Acids Res.24:5045-5047.