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Hsien-Yeh Hsu

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Hsien-Yeh Hsu , Ph.D.

Professor

 

Cornell   University

 

Personal web site

 

 

 

 

 

 

 

 

 

 

Contact Information
Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming University,

No. 155, Sec. 2, Linong St., Beitou District, Taipei City 112, Taiwan (R.O.C.)
 Phone: 02-2826-7000 ext. 7252 (Office)
              02-2826-7000 ext. 5828 (Lab)
 E-mail: hsienyeh@gmail.com 
 

 

Education and Positions Held: 

Education:    

Taiwan   University    Bachelor                         1971~1975                     Agriculture Chemistry Cornell   University   Master                               1976~1978                     Biology & Life Science

Cornell   University   Ph.D.                                 1980~1984                     Biology & Life Science

Professional:  

National   Yang-Ming   University  

Department of Biotechnology and  Laboratory Science in Medicine                   Professor            2002~present 

Institute of Biophotonics Engineering          

Adjunct Professor         2006~present 

National   Yang-Ming   University  Department of Biotechnology and  Laboratory Science in Medicine                  

Associate professor      1997~2002  

Cornell University Medical College, NY,   Department of Medicine      

Assistant Professor       1994~1997 

Cornell University Medical College, NY,  Department of Pathology

Research Associate       1991~1994 

Massachusetts Institute of  Technology, MA, Department of Biology 

Postdoctoral Associate    1990~1991 

Genzyme Inc./Gene-Trak Systems, MA, USA Research and Development 

Project Leader, Staff Scientist   1987~1990

Penicillin Assays Inc., MA, USA    Research and Development

Senior Research Scientist      1984~1987

Honors: 

Oversea Studentship to  Cornell   University , Minister of Education, , 1976  

NIH Research Award,  Cornell   University   Medical   School  ( Weill   Medical   College  of  Cornell University ), , 1993   

Academia Sinica Research Award for Junior Research Investigators, 2003  

International Society of Atherosclerosis; Investigator Award, 2003  

Research Interests:   

Investigation of the role of Extract of Ganoderma lucidum (Reishi, or Ling-Zhi, a medical fungus) polysaccharides (EORP), in T cells and in functions of regulatory T cells 

 

    Our previous studies indicated Extract of Ganoderma lucidum polysaccharides (EORP)exerts immuno-modulatory functions. The regulatory T cells (Tregs) play a bi-functional regulation between Th1 and Th2, which potentially prevent autoimmune disorders including asthma , systemic lupus erythematosus ,rheumatoid arthritis , psoriasis vulgaris . In addition, Tregs may exert anti- and anti-inflammatory. Our preliminary results indicated EORP induces naïve CD4+T cells into CD4+CD25+FOXP3+ regulatory T cells, which could inhibit effector T cells in vitro. Hence, we plan to investigate (1) Whether EORP is able to stimulate Tregs, (2) does and how EORP-mediated Tregs involve in immunological responses. Currently, using inflammatory bowel disease of mice model or other autoimmunity animal models, we will further investigate the mechanism for the role of Tregs involved in EORP alleviation of “autoimmune-like” diseases. 

The development of Extract of Ganoderma lucidum (Reishi, or Ling-Zhi) polysaccharides (EORP) to an anti-cancer drug

  Lung cancer as one of the most common cancers in  as well as in the worldrepresents a major public health problem (it is also listed as the number 1 cancer occurrence). Currently, only parts of the drugs are useful to a sub-group of patients in lung cancer, the need to develop an effective and drug in cure of lung cancer. At present, one of our projects is focused on the inhibitory effects of extract of Ganoderma lucidum (Reishi) polysaccharides (EORP) on lung cancer. The accumulated documents indicated that human immune system plays important roles in the development of lung cancer. In the collaboration with Academia Sinica and biotechnological industry, we plan to apply the functions of EORP-regulated immunity to investigate the function of human immune system in the prevention of lung cancer. In essence, we will use lung cancer cells including non-small lung cancer cells (NSLCC) bearing mice to examine the mechanism for EORP inhibition NSLCC in vitro and in vivo.  

Cordyceps sinensis (Hirsutella sinensis) mediate signalings in the regulation of cytokines expression  

  Cordyceps sinensis is considered as one of effective immune regulatory Chinese traditional medicines. Importantly, Hirsutella sinensis (HS), a true anamorph of Chinese caterpillar fungus was isolated from Cordyceps sinensis. Using the fermented HS mycelia as experimental source, we examined the effects of HS on molecular, cellular and immunological aspects of immune related cells including monocytes/macrophages and mice. In essence, we focus on pro- and anti-inflammatory related cytokines expression (e.g., IL-1, IL-1Ra, IL-10, etc.) from immune cells and mice animal model. In addition, we we’ll dissect the essential (key) components, e.g., polysaccharides, protein from HS, we will further investigate the immuno-modulator functions.Hirsutella sinensis-mediated signal transduction in regulation of cytokines expression would aid our understanding of Cordyceps-mediated human immunological activities.   

Application of molecular/cellular biology and biophotonic methods to investigate the role of lipid raft/micro-domain in receptor-mediated signallings and immune responses  

    In our recent studies, we utilized instruments of biophotonic to investigate the roles of lipid rafts in Reishi-mediated pro-inflammatory cytokine (proIL-1, IL-1) and anti-inflammatory cytokine (IL-1Ra) production within macrophages. In our previous study, we found the specific singling cascades regulated by external stimulation within macrophages are lipid raft involved. Hence we will focus on the mechanism Reishi polysaccharides activate lipid rafts and then regulate signaling cascades to enhance macrophages function. What we want to clarify is the role of Reishi polysaccharides-mediated macrophages in immune system, and we also combine various methods, which include biochemistry, molecular cell biology, and collaborated with biophotonic experts to investigate the processing of Reishi polysaccharides-mediated lipid rafts conformations, and the flow path theses lipid rafts transfer polysaccharides entering macrophages to enhance immune function. Our purpose is to understand the molecules compositions of lipid rafts which Reishi polysaccharides-induced within macrophages, and the single molecules movement (e.g. Reishi polysaccharides, TLR2, 4, etc.) by biophotonic methods to clarify the relationships between Reishi polysaccharides and macrophages.   

Selected Publications

1.           Chi-Lun Chang, Hsien-Yeh Hsu, Hong-Yu Lin, Wenchang Chiang, Hsinyu Lee. Lysophosphatidic acid-induced oxidized low-density lipoprotein uptake is class A scavenger receptor-dependent in macrophages. PROSTAGLANDINS & OTHER LIPID MEDIATORS2008;87(1-4):20-25 (SCI).(Impact factor: 1.968;Rank: 64.64%)

2.           Hsien-Yeh Hsu, Li-Chieh  Chu , Kuo-Feng Hua and Louis KuoPing Chao. Heme oxygenase-1 mediates the anti-inflammatory effect of Curcumin within LPS-stimulated human monocytes.Journal of Cellular Physiology 2008;3:603-612 (SCI).(Impact factor: 3.643;Rank: 28.21%)

 

 

3.           HSIEN-YEH HSU,* KUO-FENG HUA, WEI-CHI WU, JASON HSU, SHIH-TING WENG, TSAI-LENG LIN, CHUN-YI LIU, RUEY-SHYANG HSEU, AND CHING-TSAN HUANG. Reishi Immuno-modulation Protein Induces Interleukin-2 Expression via Protein Kinase-Dependent Signaling Pathways within human T Cells. Journal of Cellular Physiology 2008;215:15–26 (SCI).(Impact factor: 3.643;Rank: 28.21%)

4.           Louis Kuoping Chao, Kuo-Feng Hua, Hsien-Yeh Hsu, Sen-Sung Cheng I-Fan Lin, Chia-Jung Chen, Shui-Tein Chen, Shang-Tzen Chang. Cinnamaldehyde inhibits pro-inflammatory cytokines secretion from monocytes/macrophages through suppression of intracellular signaling. Food and Chemical Toxicology 2008;46:220–231 (SCI).(Impact factor: 2.186;Rank: 13.59%)

5.           Chang CL, Lin ME, Hsu HY, Yao CL, Hwang SM, Pan CY, Hsu CY, Lee H. Lysophosphatidic acid-induced interleukin-1 beta expression is mediated through Gi/Rho and the generation of reactive oxygen species in macrophages. JOURNAL OF BIOMEDICAL SCIENCE2008;15(3):357-363 (SCI).(Impact factor: 2.024;Rank: 44.44%)

6.           Feng-Ling Yang, Kuo-Feng Hua, Yu-Liang Yang, Wei Zou, Yen-Po Chen, Shu-Mei Liang, Hsien-Yen Hsu and Shih-Hsiung Wu. TLR-independent induction of human monocyte IL-1 by phosphoglycolipids from thermophilic bacteria. GLYCOCONJUGATE JOURNAL 2008;25(5):427-439 (SCI).(Impact factor: 1.602;Rank: 74.52%)

7.           魏名佐、花國鋒、許喬威、柯孟楊、曾凱雩、許先業、邱爾德. 藉由光鉗探討靈芝多醣體對於巨噬細胞的影響. 物理雙月刊 2007;廿九():1034-1039 (DI).第一或通訊作者

8.           Hsien-Yeh Hsu, Hua-Lin Wu, Sai-Koong Tan, Vivian Pei-Hsin Li, Wei-Ting Wang, Jason Hsu, and Ching-Hsun Cheng. Geldanamycin Interferes with the 90-kDa Heat Shock Protein, Affecting Lipopolysaccharide-Mediated Interleukin-1 Expression and Apoptosis within Macrophages.Molecular Pharmacology 2007;71:344–356 (SCI).(Impact factor: 4.088;Rank: 14.15%)

9.           Chien Chou, Hsien-Yeh Hsu, Hsieh-Ting Wu, Kai-Yu Tseng, Arthur Chiou, Chih-Jen Yu, Zheng-Yuan Lee and Tsu-Shin Chan. Fiber-optic biosensor for the detection of C-reactive protein and the study of protein binding kinetics. Journal of Biomedical Optics 2007;12(2):024025-1~ -9 (SCI).(Impact factor: 3.084;Rank: 4.69%)